STK39 Enhances the Progression of Cholangiocarcinoma via PI3K/AKT Pathway

Cholangiocarcinoma (CCA) is a highly aggressive form of liver cancer, but its pathogenesis far from being fully elucidated. Recent studies have reported that Serine/threonine kinase 39 (STK39) overexpressed in various tumor tissues and plays an essential role progression. However, the STK39 CCA still unclear. In this study, we investigated clinical value as well potential functions mechanisms CCA. The results showed was negatively associated with prognosis patients. Functionally, knockdown suppressed cell proliferation, migration, invasion, while overexpression facilitated aggressiveness. addition, shown to induce G2/M phase cycle arrest promote apoptosis. tumor-promoting effects were also validated by vivo experiments. Mechanistically, RNA-seq analysis identified positively regulated PI3K/AKT pathway, decreased PI3K AKT phosphorylation levels without changing total protein levels. Combined vitro experiment results, function oncogene progression activating signaling pathway. Furthermore, could gemcitabine resistance cells. Moreover, found increased expression may be mediated dysregulation miR-26a-5p. summary, study illustrates exert oncogenic through pathway served valuable prognostic candidate therapeutic target